Chromatography Samples
- 0.45µ Nylon or 0.45µ Regenerated Cellulose (RC)
For general filtration of chromatography samples prior to injection. Removal of particulates from samples is important to prevent blockage or damage to instruments. Both Nylon and RC have good solvent resistance and exhibit low extractables. Use with a pre-filter when samples contain large amounts of particulates.
Solvent Filtration and Degassing, Venting and Transducer Protection
PTFE membranes are resistant to all solvents and acids. This membrane is ideal for filtration and degassing of chromatography solvents. For sterile venting, use 0.2µ pore size, and for transducer protection or air/gas filtration, use 1.0µ or 0.45µ PTFE. PTFE is inherently hydrophobic and well suited for the removal of water vapor to protect transducers and instruments.
Cell Culture, Biological Research, Immunoblotting, Sterile Filtration
- Regenerated cellulose, cellulose acetate, polysulfone, charged nylon, nitrocellulose
Surfactant free cellulose acetate, or regenerated cellulose membranes are very low protein binding. These membranes are well suited for the sterile filtration of cell and tissue culture media, where maximum protein recovery in a filtrate is important. These filters show improved yield and performance when used with a glass pre-filter. For immunoblotting, the high protein retention of nitrocellulose and charged nylon are ideal to bind DNA.
Water and General Laboratory Filtration
- Nylon, regenerated cellulose, acrylic co-polymer
Nylon and regenerated cellulose are ideal membranes for general laboratory filtration. Nylon with 1.0µ glass pre-filter in a 50mm housing, or acrylic co-polymer in a capsule are excellent for filtration of water prior to analysis.
Syringe Filter Safety Guide
Do not exceed the pressure, temperature or chemical compatibility recommendations. High pressure can be obtained when using syringes. The smaller the sringe, the higher the pressure that can be generated. As a general guide, the following pressures can be obtained by hand with the syringes indicated:
- 20mL, 30psi;
- 10mL, 50psi;
- 5mL, 75psi;
- 3mL, 100psi;
- 1mL, 150psi;
Each user should determine the pressure they can generate by hand with a specific size syringe and take appropriate safety precautions not to exceed the recommended rating for the device used. If these limitations are exceeded, bursting of the device may occur resulting in loss of property or personal injury.
