Optimum Flow Rate, Injection Volume and Loading
Column ID
(mm)
Flow Rate
(mL/min)
Injection
Volume (µL)
Loading on
Column (µg)
2.1
0.4
1
2
3.0
0.8
3
5
4.6
1.8
5
10
Shipping Solvents
Phase Name
Solvent
Accucore RP-MS, C18, aQ, Phenyl-Hexyl, PFP Acetonitrile /Water (50:50)
Accucore HILIC
Acetonitrile /Water (90:10)
Cleaning Procedure
Before starting ensure that the solvent in the column is miscible with the cleaning
solvents. Using 50% of the working fow rate, fush the column with 20 column volumes
of each of the solvents below in the order shown.
Phase Name
Solvent
Accucore C18, RP-MS, aQ,
Phenyl-Hexyl, PFP
1. 90% Water / 10% Acetonitrile
(for buffer removal if required)
2. 50% THF / 50% Acetonitrile
3. Acetonitrile
4. Re-equilibrate with mobile phase
Accucore HILIC
1. 10% Water / 90% Acetonitrile
(for buffer removal if required)
2. 50% water / 50% Acetonitrile
3. Re-equilibrate with mobile phase
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