46
Connectors
High Pressure Capillary Tees and Crosses
MicroTee andCross for Capillary Tubing
Direct connect 1/16”, 1/32”, 360 µm OD tubing, plus other
capillary tubing
Low swept volume
Use Upchurch Scientifc
®
MicroTees andMicroCrosses to join capillary
tubing. All of these products are made entirely of PEEK
™
and have
0.006” (0.150 mm) thru-holes, with resulting swept volumes ranging
from 29 to 81 nL.
Please Note: Use only the ferrules supplied with each connector —
they are not interchangeable. Replacement ferrules and female nuts are
available on page 18. For MicroUnions, MicroTees and MicroCrosses
for UHPLC applications, see page 42.
Several researchers use our PEEK MicroTee to introduce ionizing voltage
to their fuid stream just prior to a Mass Spectrometer
1
. MicroTees are well
suited for this application due to advantageous internal geometry and PEEK
polymer’s electrical resistance. The materials required for this setup are as
follows: one gold or platinum conducting wire, one P-775 or P-875 MicroTee
(this page), one MicroTight Tubing Sleeve (page 19) for the conducting wire
(as needed to accomodate wire diameter), and at least two more MicroTight
Tubing Sleeves (page 19) to connect your capillary tubing.
Connect the wire to the center port of the MicroTee. First, thread your wire
through the appropriate tubing sleeve, if necessary, with the wire extending
beyond both ends of the sleeve. Slip the female nut included with the
MicroTee over the wire or sleeved wire, followed by the ferrule — ensuring
the wire (and its sleeve) extends well past the end of the ferrule tip. Align the
tip of the wire with the thru-hole of the MicroTee and gently insert the wire
until it bottoms out. Now fnger tighten the female nut into place. Attach your
fow path tubing to the MicroTee’s two other available ports, following the
instructions provided with the MicroTee.
Begin fuid fow through the tee and apply voltage to the conducting
wire lead. This setup typically provides effective electrospray ionization in
applications having a fow rate of 100 µL/min or greater.
1
One such paper describing pioneering electrospray work:
Protein Identifcation at the Low Femtomole
Level fromSilver-StainedGels Using a NewFritless Electrospray Interface for Liquid Chromatography-
Microspray and Nanospray Mass Spectrometry.
Christine L. Gatlin, Gerd R. Kleemann, Lara G. Hays,
Andrew J. Link, John R. Yates III (1998) Analytical Biochemistry 263, 93-101.
Application Note
Part No. Description
Threads
Includes
Thru-hole
Swept Volume Pressure Rating
MICROTEE, MICROCROSS AND MICROELBOW
P-775 PEEK MicroTee for MicroTight Sleeves
5/16-24 Coned
(3) F-172, (3) P-416
0.006” (0.150 mm) 29 nL
4,000 psi (276 bar)
P-777 PEEK MicroCross for MicroTight Sleeves
5/16-24 Coned
(4) F-172, (4) P-416
0.006” (0.150 mm) 38 nL
4,000 psi (276 bar)
P-875 PEEK MicroTee with Mounting Hole, for MicroTight Sleeves 5/16-24 Coned
(3) F-172, (3) P-416
0.006” (0.150 mm) 29 nL
4,000 psi (276 bar)
P-885 PEEK MicroTee for 1/32” OD Tubing
5/16-24 Coned
(3) F-112, (3) P-416
0.006” (0.150 mm) 29 nL
5,000 psi (345 bar)
P-887 PEEK MicroCross for 1/32” OD Tubing
5/16-24 Coned
(4) F-112, (4) P-416
0.006” (0.150 mm) 38 nL
5,000 psi (345 bar)
P-888 PEEK MicroTee for 360 µm OD Tubing
5/16-24 Coned
(3) F-152, (3) P-416BLK 0.006” (0.150 mm) 29 nL
5,000 psi (345 bar)
P-889 PEEK MicroCross for 360 µm OD Tubing
5/16-24 Coned
(4) F-152, (4) P-416BLK 0.006” (0.150 mm) 38 nL
5,000 psi (345 bar)
P-890 PEEK MicroTee for 1/16” OD Tubing
5/16-24 Coned
(3) F-132, (3) P-416
0.006” (0.150 mm) 58 nL
5,000 psi (345 bar)
P-891 PEEK MicroCross for 1/16” OD Tubing
5/16-24 Coned
(4) F-132, (4) P-416
0.006” (0.150 mm) 81 nL
5,000 psi (345 bar)
P-888
MicroTee
for 360 µm OD tubing
0.006” thru-holes
with fttings included
P-890
MicroTee
for 1/16” OD tubing
0.006” thru-holes
with fttings included
1.19”
(3.02 cm)
1.19”
(3.02 cm)
0.80”
(2.03 cm)
0.80”
(2.03 cm)
P-775
MicroTee for MicroTight
®
Tubing Sleeves
0.006” thru-holes
with fttings included
(tubing sleeves not included)
P-887
MicroCross
for 1/32” OD tubing
0.006” thru-holes
with fttings included
1.19”
(3.02 cm)
0.80”
(2.03 cm)
1.19”
(3.02 cm)
1.19”
(3.02 cm)
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