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Comments
There
are many materials that can block injector passages, including crystallized
buffer salts, suspended matter in the sample or mobile phase, burrs from
tubing and fittings, and particles from pump seals.
The following
routine procedures will prevent blockage:
1) Filter particles from the mobile phase and, when necessary, the samples.
2) Install a filter between the pump and injector to protect the injector
from wear particles from the pump.
3) Rinse the injector periodically, especially before shutting down, when
using buffers for mobile phase or sample solvent.
4) Use burr-free tubing and fittings to avoid metal shavings.
Blockage — High Back Pressure
The following pages discuss the causes of complete or partial blockage in the injector. The problem has two cases. 1) The loop is difficult to load. In this case, the system pressure (pressure at the pump) may be either normal or high. 2) The loop is not difficult to load, but the system pressure is high. These problems are most commonly encountered when using buffer salts in the mobile phase.
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