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Comments The normal procedure for flushing the needle port is: Use about 1 mL of mobile phase. Use a Needle Port Cleaner (Rheodyne Part Number 7125-054). Flush only when the handle is in the INJECT position, so flow goes directly out vent #5 and bypasses the loop. If you have no cleaner, use a luer tip syringe. See Fig. 18 in Symptom #5. In solving Causes A and B, make an additional flush when the handle is in LOAD in order to flush the passages of ports #4 and #6, and vent tube #6. If the purpose of flushing is to remove buffer salts, do it with water not organic solvents. In routine operation, remove the syringe after turning to INJECT. However, keep the handle in this position so the loop is continuously flushed with mobile phase. We recommend keeping the needle port filled with fluid by flushing every ten or twenty injections. Flush just before the next analysis. Backflushing ports with the pump set at high flow rate is sometimes effective in cleaning certain passages. See also Symptom #9 for related problems. |
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