Page 10 - HAI_Price_List_2010

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HA I S I L 3 0 0

HAISIL 300 For Biomolecules

Phases C18 Par ticle Sizes 5 and 10µm* Pore Size 300Å Pore Volume 0.7mL/gm Surface Area 80m 2 /gm %Carbon (w/w) C18 = 6%

Phase type Monofunctional and fully endcapped Silica Class Type A

Applications

HAISIL 300 columns and cartridges are ideal for biomolecule separations such as peptides and small proteins. Optimized phase bonding and column packing techniques assure unsurpassed perform-ance and column-to-column

reproducibility. Shor t car tridge columns are economical and are ideally suited for LC/MS applications. The column and cartridge offering ranges from 150µm to 20mm I.D. which means that biosepara-tions can be optimized by selecting a format that best fits your analytical requirements.

Micro-Preparative HPLC

Higgins Analytical Car tridge Columns are helping researchers understand the immune response of living cells. An inves-tigation to understand the genetic con-trol of the immune response systems in organisms involves the extraction, isola-tion, and identification of more than 10,000 peptides. These distinct peptides are bound to a major histocompatibility complex (MHC) molecule on tumor cells.

Extraction of approximately 5x10

10 cells yield several nanomoles of a very com-plex peptide mixture. Several rounds of RP-HPLC with different organic modifiers on a 40x2.1mm PEEK car tridge column (HAISIL 300 C18 5µ m, P/N: HK-0421-W185) are performed to fractionate the mixture into individual components.

The chromatograms on the left illustrate how this technique is being used to nar-row the search for the one peptide that marks a par ticular tumor cell as being diseased enabling it to be recognized by the immune cells.

Fractionation of a Purified Tumor Cell Extract

Peptides ranging from 100 fmol to 1pmol are collected in 40sec intervals during a 70min gradient from 0 - 60% MeCN with HFBA modifier. A 40x2.1mm HAI SIL 300 C18 5µm cartridge column (HK-0421-W185) is used.

Reanalysis of a Fraction from the Chromatogram above

The same 40x2.1mm HAI SIL 300 cartridge is used for a 70min gradient from 0-60% MeCN withTFA modifier. Tic marks indicate

40sec fraction collections. Peek and Titanium car tridge columns

used in the application described above. P/N: HK-0421-W185

Guide to HAISIL 300 Part Numbers

Hx-xxxx-W185 HAISIL 300 C18 5µm

See Page 23 for complete Part Number information

* 10µm is offered in HAISpeed 20 Guard Cartridges only. See pages 68-69.