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Enzymatic Reaction Mixtures of Human 15-lipoxygenase

HAISIL 100 C18 250x4.6mm 74.9% MeOH, 25% water, 0.1%HoAC

1mL/min, 234nm P/N HS-2546-M185

Economical Alternative for PICOTAG Analysis

Our HAISIL 100 C18 columns have demonstrated superior resolution and life time for PICOTAG™ amino acid analysis when compared to considerably more expensive columns from other manufac-turers. Besides good linearity and column lifetime, the 150x4.6mm column yields very efficient amino acid analysis as shown in the chromatogram above (P/N HS-1546-M185).

Conditions

HAISIL 100 C18 5µm 150x4.6mm Solvent A: Phosphoric acid, ammonium

sulfate aqueous buffer Solvent B: Acetonitrile. Linear gradient at 1mL/min 254nm detection

Analogs:

A1: pGlu-His-Trp-Ser-Tyr-(d-Ser(but))-Leu-Arg-Pro-NHEt

A2: pGlu-His-Trp-Ser-Tyr-d-Ala-Leu-Arg-Pro-NHEt A3: pGlu-His-Trp-Ser-Tyr-d-Leu-Leu-Arg-Pro-NHEt

A4: pGlu-His-Trp-Ser-Tyr-d-Ala-Trp-Leu-Pro-NHEt A5: pGlu-His-Trp-Ser-Tyr-d-Trp-Leu-Arg-Pro-NHEt

Hormone Analogs

LH-RH (Luteinizing Hormone - Releasing Hormone) Analogs are used for the inhibition of gonadal steroid production. An impor tant application area is in the treatment of prostate cancer.

Kinetic Measurements in Disease Research

Researchers at the University of California, Santa Cruz have recently published a novel HPLC method that yields baseline separation of per-deuter-ated and protonated lipoxygenases.

The striking ability of a 250x4.6mm HAISIL 100 C18 column to resolve these two very similar compounds is enabling researchers to study large competitive kinetic isotope effects in Human 15-lipoxygenase catalysis. Protio/per-deutero ratio data obtained by this HPLC method agreed within experimental error of data obtained by ES-MS.

The elucidation of these mechanisms is noteworthy because of the increasing role human lipoxygenases are playing in inflammatory diseases and cancer growth regulation.

Alkaloids

A Peruvian vine named “Una de Gato” (Uncaria tomentosa) and commonly referred to as Cat’s Claw, has become a popular natural product source for herbal remedies. Enthusiasts claim the oxindole alkaloid extracts to be effective in the treatment of a wide range of digestive and circulatory diseases as well as other afflictions ranging from cancer to arthritis. Reputable providers of such phytochemical containing formulations carefully assay the alkaloid content of the extracts and formulations. The chromatogram above illustrates baseline resolution of Cat’s Claw bark extracts containing isopteropodine, pteropodine, mitraphylline, isomitraphylline, ryncophylline and isorynchophylline.

Alkaloids from Cat’s Claw

analyzed on a HAISIL 100 C18 column

P/N HS-2546-M185

HAISIL 100 - the Separations Work Horse

The 100Å pore size, monomeric bonded phase, and very high performance make HAISIL 100 an ideal choice for separa-tions ranging from small molecules such as carbamate pesticides and pharmaceu-

tical drugs to synthetic oligonucleotides and small peptides. High efficiency, symmetrical peaks, and long column life is characteristic not only of the C8 and C18 reverse phase columns, but the normal phase silica columns as well.

Carbamate Pesticide Analysis

250 x 4.6mm HAISIL 100 C18

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