Page 16 - HAI_Price_List_2010

14

P ROTO2 0 0

Applications

300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.

200

PROTO 200 150x4.6mm /N/ RS-1546-D185

238EV 150x4.6mm

218TP 250x4.6mm

Phosphopeptide Purity Analysis

Peptide HPLC column performance, using the same gradient conditions, was evaluated on the PROTO™ 200 C18, a new peptide analysis column obtained fromThe Nest Group, Inc.. Against the industry standard, Grace/Vydac® 218TP C18, and their newer peptide chemistry, Everest® C18, the data show that the PROTO™ 200 C18 column has superior resolution to the 218TP chemistry and better resolution, especially for the more hydrophobic peptides, than the Everest® C18. In addition, the PROTO 200 columns can be run without TFA, which allows better detection when utilizing LC-MS.

Previously, the Everest chemistry had been shown by Grace/Vydac to be superior to all other wide pore columns, especially for the more hydrophobic peptides. However, the ready availability of the PROTO™ 200 peptide columns, in either C4 or C18 chemistries, and in capillary through preparative sizes, now allows researchers their first oppor tunity to save both time and money on a wider variety of applications, compared to the Grace/Vydac columns, and by extension to all other peptide columns.

Comparison of Phosphopeptide Purity Analysis

PROTO ™ 200 More Selectivity and

Resolution for Peptides

Phases C4 and C18 Particle Sizes 4.5 and 10µm Pore Size 200Å Pore Volume 1.1mL/gm Surface Area 200m 2 /gm

%Carbon (w/w) C4. = 4.5%, C18= 14%

Phase type Monofunctional & fully endcapped Silica Class Type B

Guide to PROTO 200 Part Numbers

Rx-xxxx-D045 PROTO 200 C4 5µm Rx-xxxx-D185 PROTO 200 C18 5µm

See Page 23 for complete Part Number information

200