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Proto™ 200 C18 provides greater selectivity than the popular 300Å choice

Tryptic Digest of Carbamidomethylated BSA (16h, 20 pMols on column).

Column Size: 150 x 1.0mm

Column Brand:

Upper Chromatogram: Vydac 300Å 238MS C18 5µm P/N 238MS5115 Lower Chromatogram: Higgins 200Å Proto 200 C18 5µm P/N RS-1501-D185

Conditions:

A = 0.1% formic acid containing 0.01% TFA

B = 0.085% formic acid in CH3CN containing 0.01% TFA

Initial 5 min isocratic hold at 5% B, then 60 min hyperbolic gradient to 65%B Flow 0.15 ml/min, Green & Black curves: Blank gradient profile

Higgins Analytical thanks Amos Heckendorf of The Nest Group (www.nestgrp.com) for these Proto 200 data.

200