182
Column Hardware
Iso-Prep
™
Guard
21.2 mm and larger ID column protection
Improves plate count and symmetry
Simple manufacturing technique
Guard Columns
Part No.
Description
ISO-PREP GUARD ORDERING INFORMATION
9197-P
Iso-Prep Guard Holder
9197-20
Iso-Prep Guard Cartridge, 21.2 mm x 1 cm, 1 Frit
8056
Iso-Prep Guard, 21.2 mm Frit
9197-S
Iso-Prep Guard Finishing Tool
SEMI-PREP GUARD COLUMN
10 mm ID x 1 cm
C-1000
Semi-Prep Cartridge Guard Column Holder
C-1033
Dry Packing Funnel
C-1035
Semi-Prep Cartridge
REPLACEABLE CARTRIDGE GUARD COLUMN FRIT CAPS
C-1030
Threaded Frit Cap with 2 µm Stainless Steel Frit
C-1031
Threaded Frit Cap with 2 µm Titanium Frit
Application Note
Why use guard columns?
Columns damaged by strong or irreversible adsorption include changes
in retention time and selectivity, offset baseline (bleed) and peak shape
deterioration. Columns damaged by particulates (from the sample matrix,
mobile phase, etc.) commonly exhibit peak splitting and increased back
pressure. Guard columns protect your column in two ways. First, they act
as flters trapping particles in their frits or packed bed, or both. Second,
when the guard column is packed with the same material as in the analytical
column, it removes compounds that irreversibly or strongly bind to the
packing material. By either approach, guard columns can increase your
column life considerably.
UnpackedSemi-PrepGuardColumn
10 mm ID column protection
Convenient cartridge system
Easy to pack
The internal volumeof this Upchurch Scientifc
®
semi-prepguard column
is just 780 µL, which only requires approximately 1.50 g of packing
material — ideally the same material
used in your semi -prep column.
The C-1000 Holder will hold to high
pressures, and is specially treated to
prevent galling.* Use standard 10-32
coned fttings (not included) to connect
your 1/16” OD tubing.
* Galling is a form of “cold welding.” When two fttings
manufactured from the same metal are wrench-tightened
too tightly, they can “weld” together, making it virtually
impossible to separate the two components.
C-1000
Semi-Prep Cartridge
Guard Column Holder
1.44”
(3.66 cm)
1.00”
(2.54 cm)
C-1000 Semi-Prep
Guard Column
Fittings, tubing and column shown
are not included.
Low Pressure Drop
60:40 Acetonitrile : Water
50 mL/min
Kromasil 10 µm C18
Backpressure: 100 psi
Without Iso-Prep Guard — 38,150 Plates/M 1.24 As
10 μ C18 100 x 21.2 mm, 60:40 Acetonitrile:Water, 20 mL/min
With Iso-Prep Guard — 41,920 Plates/M 1.20 As
Application Note
FOR ORDERING & DISTRIBUTOR INFORMATION +1 800 426 0191 • +1 360 679 2528 • www.idex-hs.com