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23 gauge
Microseal compatible SPME probes:
blunt cone
shape on tip
23 gauge
(0.63 mm, 0.025 inches)
Preferred:
Required:
Microseal compatible syringe needles:
Do not use:
sharp, slicing tip
sharp pointed tip
tapered dual gauge 23-26
24 gauge SPME probe
1079
1177
Microseal Installation
1. Follow the standard procedure to prepare the instrument
for a septum change.
2. Remove the standard injection port nut and the septum.
Remove any pieces of septum that are stuck to the injection
port. Clean or replace the injection port liner to remove
any septum particles which may have accumulated from
silicone rubber septa.
3. If your instrument requires a Microseal adapter, mount it
on the injection port finger-tight.
4. Place the Microseal in the septum cup. Push it down gently
with your finger to seat it in the septum cup.
5. Screw on the Microseal nut finger-tight. Do not overtighten.
Choosing a Syringe
The Microseal only requires the correct 23 gauge syringe
needle diameter and needle tip shape shown below to function
properly. Syringes for autosamplers need the correct needle
length, syringe barrel, and plunger configuration for compatibility.
Microseals also work well with manual injection including the
Merlin Microshot. Further information about Microseal syringe
selection, including links to tables of recommended syringe part
numbers is available at www.merlinic.com/products/merlin-
microseal/syringes-for-microseal.
Injection Port Adapters for Microseals
All Microseals are compatible with all adapters. The table at
the end of this manual lists Merlin part numbers for all adapters
and Microseals. Additional information about individual adapters
can be viewed at
www.merlinic.com/products/merlin-microseal.
Agilent
Microseals fit directly in the Agilent
septum cup and require only a Merlin
Microseal nut. No additional adapter is
required.
PerkinElmer
The Microseal adapter for the
PerkinElmer injection ports mounts directly
to the port. The Microseal nut has the
correct autosampler alignment detail.
Shimadzu
AMicroseal adapter is available for
injection ports on Shimadzu Models
GC-2010 and GC-2025. The
Microseal nut has alignment details
for the Shimadzu AOC-20 series and
AOC-5000 autoinjectors.
Bruker/Varian
Separate Microseal adapters are available for 1079 and
1177 Bruker/Varian injection ports. The Microseal nuts
incorporate the microswitch start assemblies.
Sharp edged or sharp pointed needles can pierce and damage
the duckbill seal. The 23/26 gauge tapered needles and 24 gauge
SPME probes will not seal properly in the Microseal.
Thermo Scientific
Thermo Scientific Company has designed their own
Microseal adapters. An adapter kit for PTV and S/SL injection
ports is available directly from Thermo Scientific authorized
distributors. Also available from Thermo Scientific are kits to
align their autosamplers with their Microseal adapters. More
information is available at www.merlinc.com/products/merlin-
microseal/thermo-port-adapter.